Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
Product parameters:
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Kit composition (WLRE8208KIT 48T/Pack)
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Composition
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Content
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| E buffer |
1 mL×2 Tubes
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| B buffer |
150 μL×1Tube
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Positive control template
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100 μL×1Tube
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| Reagents |
48T
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Product features and advantages:
| Product features and advantages |
| Features |
Advantages |
| High sensitivity |
1 copies/ml |
| Strong specificity |
Specificity is superior to PCR
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| Short reaction time |
20min |
| Polymorphic reagent |
Liquid, freeze-dried powder, freeze-dried ball
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| Easy to operate |
Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the results
Design of primers and probe is simple
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| Easy to store and transport |
It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.
The freeze-dried form has a long storage time and is convenient for transportation
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Low requirements on equipment
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Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;
Applicable to our WL-16-III and other isothermal fluorescence detector
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Product application:
| Application |
| Ultra-clean laboratory |
African Swine Fever Virus Isothermal Detection
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| Indoor |
Operation procedure and process:
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Operation procedure and process:
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Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix.
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Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent.
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Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid
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Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control
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Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap)
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Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone.
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Performence Comparison Data:
| No. |
TT value |
Template concentration |
Result determination
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| 1 |
4.2 |
10-1 |
Positive |
| 2 |
5.5 |
10-2 |
Positive |
| 3 |
10.2 |
10-3 |
Positive |
| 4 |
17.0 |
10-4 |
Positive |
| 5 |
12.0 |
10-5 |
Positive |
| 6 |
- |
10-6 |
Negative |
| 7 |
- |
10-7 |
Negative |
| 8 |
Negative control |
Negative |
Support and Services:
Livestock Disease Kit Technical Support and Services
We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.
- Our technical support staff is available to answer any questions you may have about the product.
- We offer online tutorials and user guides to help you understand the product better.
- We provide technical assistance to help troubleshoot any issues you may be having with the product.
- We offer a warranty on our products.
If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.
FAQ:
- Q: What diseases can Livestock Disease Kit detect?
A: Livestock Disease Kit can detect different infectious diseases, such as Foot and Mouth Disease, Bluetongue Disease, Bovine Viral Diarrhea.
- Q: How does Livestock Disease Kit work?
A: Livestock Disease Kit uses advanced MIRA technology to amplify the target DNA/RNA targent sequences , and detect the disease by analyzing the amplified sequences.
- Q: How long does it take to get results from Livestock Disease Kit?
A: Livestock Disease Kit can give a result within 5-20 mins.
- Q: What type of sample is suitable for Livestock Disease Kit?
A: Livestock Disease Kit is suitable for blood, serum, and tissue samples.
- Q: Does Livestock Disease Kit require professional equipment?
A: No, Livestock Disease Kit does not require any professional equipment,can use with our isothermal equipment or pcr machine
