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NFO Isothermal PCR Kit Room Temp High Accuracy 14 Months

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Amp-Future (Changzhou) Biotech Co., Ltd.

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NFO Isothermal PCR Kit Room Temp High Accuracy 14 Months

Name: NFO Isothermal PCR Kit Room Temp High Accuracy 14 Months
Brand: Amp-future bio
Price: 3.8 USD

Brand Name : Amp-future bio

Model Number : WLN8210KIT

Certification : ISO9001

Place of Origin : China

MOQ : 48T

Price : USD$3.8/T

Payment Terms : T/T, MoneyGram

Supply Ability : 100000T/Month

Delivery Time : 6 working days

Packaging Details : 16T/Bag,48T/Box

Application : DNA Nucleic acid

Format : NFO Kit

Kit Components : Reagents,Buffer,Enzymes

Manufacturer : Amp-future bio

Product Name : DNA Isothermal Amplification Kit NFO

Reaction Time : 5-20mins

Reaction Volume : 50μL

Sensitivity : 500-1000copies/ml

Shelf Life : 14 months

Specificity : High

Storage Conditions : 5-20℃

Suitability : Universal

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Isothermal NFO Amplification Kit | Room Temp & High Accuracy | 14 Months

`Product Detail`

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3' end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

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