Room Temperature Isothermal PCR Kit Constant Temp Freeze Dried Reagents

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Amp-Future (Changzhou) Biotech Co., Ltd.

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Room Temperature Isothermal PCR Kit Constant Temp Freeze Dried Reagents

Name: Room Temperature Isothermal PCR Kit Constant Temp Freeze Dried Reagents
Brand: Amp-future bio
Price: 3.8 USD

Brand Name : Amp-future bio

Model Number : WLRN8211KIT

Certification : ISO9001

Place of Origin : China

MOQ : 48T

Price : USD3.8$/T

Payment Terms : T/T paypal

Supply Ability : 100000T/Month

Delivery Time : 6days

Packaging Details : 16T/Bag,48T/Box

Application : RNA Nucleic Acid Amplification

Components : Primers, Taq DNA Polymerase, dNTPs, Mg2+

Detection Method : NFO

Instrument : Test strips

Kit Size : 48 reactions

Product Format : Lyophilized

Product Name : RNA Isothermal Amplification NFO Kits

Product Type : Kit

Reaction Time : 5-20 minutes

Reaction Volume : 50μl

Shelf Life : 14 months

Shipping Condition : Ice

Storage Temperature : -20°C

Target Template : RNA

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`Product Detail`

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3' end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	RNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for RNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

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